产品货号：
GS4564
中文名称：
重组小鼠M-CSF
英文名称：
Recombinant Murine Macrophage Colony Stimulating Factor
产品规格：
2μg|10μg
发货周期：
1～3天
产品价格：
询价
General Description
Macrophage Colony Stimulating Factor (M-CSF),also named CSF-1,is a hematopoietic growth factor that is involved in the proliferation,differentiation,and survival of monocytes,macrophages,and bone marrow progenitor cells.It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R.M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer.Four transcript variants encoding three different isoforms have been reported for M-CSF gene.Although forms may vary,all of them contain the N-terminal 150 a.a.portion that is necessary and sufficient for interaction with the receptor.The first 229 a.a.of mature mouse M-CSF shares 87%,83%,82% and 81% sequence identity with corresponding regions of rat,dog,cow and human M-CSF,respectively.Human M-CSF is active in the mouse,but mouse M-CSF is reported to be species-specific.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.
Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
Molecular Weight
Approximately 52.0kDa,a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains.
Purity
＞95% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2ng/ml,corresponding to a specific activity of> 5.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in 20mM Tris,500mM NaCl,pH7.4.
Endotoxin
Less than 1 EU/μg of rMuM-CSF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should
be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
AA Sequence
KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS
CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN
TPNANATERL QELSNNLNSC FTKDYEEQNK
ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN
NSFAKCSSRD VVTKPDCNCL YPKATPSSDP
ASASPHQPPA PSMAPLAGLA WDDSQRTEGS
SLLPSELPLR IEDPGSAKQR PPRSTCQTLE
相关搜索：重组小鼠M-CSF，M-CSF，小鼠M-CSF，重组M-CSF，CSF-1
Macrophage Colony Stimulating Factor (M-CSF),also named CSF-1,is a hematopoietic growth factor that is involved in the proliferation,differentiation,and survival of monocytes,macrophages,and bone marrow progenitor cells.It is produced by osteoblasts (as a result of endocrine stimulation by parathyroid hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R.M-CSF is a four α-helical bundle cytokine and its active form is found extracellularly as a disulfide-linked homodimer.Four transcript variants encoding three different isoforms have been reported for M-CSF gene.Although forms may vary,all of them contain the N-terminal 150 a.a.portion that is necessary and sufficient for interaction with the receptor.The first 229 a.a.of mature mouse M-CSF shares 87%,83%,82% and 81% sequence identity with corresponding regions of rat,dog,cow and human M-CSF,respectively.Human M-CSF is active in the mouse,but mouse M-CSF is reported to be species-specific.
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.
Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Usage
This material is offered by bjbalb Biotech for research,laboratory or further evaluation purposes.NOT FOR HUMAN USE.
Molecular Weight
Approximately 52.0kDa,a disulfide-linked homodimer consisting of two 230 amino acid polypeptide chains.
Purity
＞95% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine M-NFS-60 cells is less than 2ng/ml,corresponding to a specific activity of> 5.0×105IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered solution in 20mM Tris,500mM NaCl,pH7.4.
Endotoxin
Less than 1 EU/μg of rMuM-CSF as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should
be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
AA Sequence
KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS
CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN
TPNANATERL QELSNNLNSC FTKDYEEQNK
ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN
NSFAKCSSRD VVTKPDCNCL YPKATPSSDP
ASASPHQPPA PSMAPLAGLA WDDSQRTEGS
SLLPSELPLR IEDPGSAKQR PPRSTCQTLE
相关搜索：重组小鼠M-CSF，M-CSF，小鼠M-CSF，重组M-CSF，CSF-1